Figure 3.

(a) Time-dependent modification of Hb A in normal blood incubated with 2 mM test compounds. (b) Concentration-dependent inhibition of SS cell sickling under hypoxia for 2 h. (c) Concentration-dependent modification of Hb S incubated with test compounds. Hemolysates from the above antisickling assay were subjected to cation-exchange HPLC analyses and levels of modified Hb were assessed. (d) Concentration-dependent P ₅₀ shift of Hb S incubated with test compounds. Hemolysates from the antisickling assay were used for the study.