Skip to main content
. 2018 Sep 6;174(6):1450–1464.e23. doi: 10.1016/j.cell.2018.07.002

Figure 2.

Figure 2

Nrx HS Modification Is Required for Development of Functional Synapses in Cultured Hippocampal Neurons

(A) Density of excitatory synapses (apposed PSD-95-VGluT1 puncta) was not significantly altered by Nrx knockdown.

(B) Density of inhibitory synapses (apposed gephyrin-VGAT puncta) was reduced by Nrx knockdown and rescued by RNAi-resistant Nrx∗ but not Nrx∗ΔHS.

(C and D) Excitatory and inhibitory transmission were impaired by Nrx knockdown and rescued by RNAi-resistant Nrx∗, but not Nrx∗ΔHS. All inter-event interval distributions were significantly different except that shCon+CFP versus shNrx+Nrx∗, and shNrx+CFP versus shNrx+Nrx∗ΔHS, did not differ for mIPSCs; comparing Nrx∗ΔHS with Nrx∗ rescue gave p < 0.05 for mEPSCs and p < 0.001 for mIPSCs by Kruskal-Wallis and Dunn’s tests. Amplitude data are shown in Figure S3C.

∗p < 0.05, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 by Kruskal-Wallis and Dunn’s tests compared to shCon+CFP; n = 39–55 (A and B), 21–32 (C), or 18–20 (D) from 3–4 independent experiments. shNrx plus Nrx∗ΔHS not significant compared with shNrx plus CFP for all assays. Error bars represent SEM. Scale bars: 5 μm.

See also Figure S3.