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. 2018 Oct 1;198(7):914–927. doi: 10.1164/rccm.201707-1497OC

Figure 5.

Figure 5.

PTPN13 (protein tyrosine phosphatase-N13) decreases Fas tyrosine phosphorylation and Fas-induced apoptosis. (A) Western blot depicting the time-dependent increase in phosphorylation of Fas at Y291 after tumor necrosis factor-α/IFN-γ sensitization. (B) Western blot analysis showing increased tyrosine phosphorylation of Fas at Y291 after siRNA1-mediated PTPN13 knockdown. (C) Western blot showing increased phosphorylation of Fas at Y291 after treatment with acylated-SLV peptide. (D) Western blot analysis illustrating increased phosphorylation of Fas at Y291 after pervanadate treatment. (E) Caspase-8 activation in MRC5 myofibroblasts is significantly increased after pretreatment with pervanadate for 2 hours before stimulation with agonistic anti-Fas antibody. ***P < 0.001, mean ± SEM . n = 3. PBS = phosphate-buffered saline; TNF = tumor necrosis factor.