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. 2017 Aug 20;42(3):97–104. doi: 10.1584/jpestics.D17-006

Fig. 2. Expression of A. gossypii CYP6CY22 and CYP6CY13 genes in transfected D. melanogaster S2 cells. Lane 1=molecular weight standards. Lanes 2 and 5=negative control, cells transfected only with the pAc5.1 vector without inserted genes. Lanes 3 and 4=cells transfected with c21228_g1 and c21368_g1 genes and cultured in normal medium (Schneider’s Drosophila Medium with 10% FBS). Lanes 6 and 7=cells transfected with c21228_g1 and c21368_g1 genes (59 kDa) and cultured in CYP expression medium (Insectagro DS2 medium supplemented with 0.1 mM ferric citrate, 0.1 mM 5-aminolevulinic acid, and 4 mM Ala-Glu without FBS).

Fig. 2. Expression of A. gossypii CYP6CY22 and CYP6CY13 genes in transfected D. melanogaster S2 cells. Lane 1=molecular weight standards. Lanes 2 and 5=negative control, cells transfected only with the pAc5.1 vector without inserted genes. Lanes 3 and 4=cells transfected with c21228_g1 and c21368_g1 genes and cultured in normal medium (Schneider’s Drosophila Medium with 10% FBS). Lanes 6 and 7=cells transfected with c21228_g1 and c21368_g1 genes (59 kDa) and cultured in CYP expression medium (Insectagro DS2 medium supplemented with 0.1 mM ferric citrate, 0.1 mM 5-aminolevulinic acid, and 4 mM Ala-Glu without FBS).