Table 2. Selected genes identified by RNA-seq that were differentially transcribed at a significant level between the insecticide-resistant A. gossypii clone (KR) and the insecticide-susceptible clones (NS and MS).
| Gene IDa) | CDS length (bp) | ID of top-hit NCBI-nr protein | Description of top-hit NCBI-nr protein | Identity of top-hit (%) | Fold change of expression level | No. of tag countsb) | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| KR/NS (N) | KR/MS (N) | KR/NS (T) | KR/MS (T) | NS (N) | NS (T) | MS (N) | MS (T) | KR (N) | KR (T) | |||||||||||||||||
| rep1 | rep2 | rep3 | rep1 | rep2 | rep3 | rep1 | rep2 | rep3 | rep1 | rep2 | rep3 | rep1 | rep2 | rep3 | rep1 | rep2 | rep3 | |||||||||
| c21228_g1 | 1542 | XP_001948421 | PREDICTED: cytochrome P450 6k1-like [Acyrthosiphon pisum] | 78.35 | 46.21 | 4.35 | 38.05 | 3.71 | 53 | 10 | 23 | 50 | 23 | 25 | 411 | 374 | 262 | 612 | 542 | 243 | 1,166 | 1,654 | 943 | 1,516 | 1,086 | 1,521 |
| c21368_g1 | 1542 | XP_008184269 | PREDICTED: probable cytochrome P450 6a13 [Acyrthosiphon pisum] | 80.39 | 7.46 | 3.32 | 8.22 | 2.48 | 221 | 152 | 223 | 186 | 138 | 156 | 815 | 405 | 394 | 694 | 797 | 682 | 1,422 | 2,003 | 870 | 1,975 | 1,126 | 1,278 |
| c29523_g2 | 420 | XP_008184269 | PREDICTED: probable cytochrome P450 6a13 [Acyrthosiphon pisum] | 74.81 | 5.17 | 2.11 | 1.19 | 0.56 | 52 | 29 | 29 | 86 | 26 | 57 | 126 | 135 | 51 | 219 | 231 | 51 | 312 | 78 | 210 | 81 | 84 | 61 |
| c22273_g1 | 1533 | XP_001946384 | PREDICTED: probable cytochrome P450 6a13 [Acyrthosiphon pisum] | 91.59 | 3.68 | 9.06 | 1.21 | 1.99 | 161 | 153 | 168 | 83 | 50 | 79 | 80 | 72 | 77 | 61 | 62 | 52 | 598 | 429 | 752 | 65 | 119 | 99 |
| c26291_g2 | 1536 | XP_001952450 | PREDICTED: probable cytochrome P450 6a13 [Acyrthosiphon pisum] | 76.45 | 3.01 | 19.84 | 2.99 | 1.99 | 2,111 | 2,289 | 2,422 | 134 | 79 | 94 | 332 | 312 | 555 | 219 | 223 | 191 | 7,763 | 4,927 | 8,128 | 312 | 340 | 363 |
| c26291_g1 | 1518 | XP_001952450 | PREDICTED: probable cytochrome P450 6a13 [Acyrthosiphon pisum] | 81.98 | 2.66 | 45.25 | 1.93 | 1.88 | 465 | 384 | 546 | 52 | 27 | 29 | 44 | 28 | 26 | 55 | 78 | 21 | 1,727 | 341 | 1,842 | 67 | 71 | 92 |
| c4974_g1 | 1533 | XP_008188450 | PREDICTED: uncharacterized protein LOC100168454 [Acyrthosiphon pisum] | 88.34 | 2.43 | 3.94 | 5.39 | 1.84 | 478 | 407 | 484 | 153 | 112 | 95 | 502 | 218 | 296 | 629 | 476 | 352 | 1,188 | 1,158 | 943 | 768 | 729 | 663 |
| c27644_g1 | 1554 | XP_001947920 | PREDICTED: cytochrome P450 6a2-like [Acyrthosiphon pisum] | 74.81 | 1.59 | 4.26 | 3.43 | 0.83 | 785 | 699 | 974 | 113 | 56 | 72 | 490 | 413 | 189 | 622 | 665 | 112 | 1,495 | 1,015 | 1,447 | 310 | 255 | 347 |
| c23819_g1 | 288 | XP_001951034 | PREDICTED: cytochrome P450 4C1-like [Acyrthosiphon pisum] | 78.12 | 0.48 | 0.79 | 3.18 | 3.16 | 5 | 5 | 2 | 6 | 2 | 3 | 6 | 2 | 1 | 5 | 2 | 8 | 7 | 0 | 0 | 8 | 24 | 8 |
a) The amino acid sequence identities of each gene compared with Acyrthosiphon pisum P450 sequences acquired from the NCBI-nr protein sequence database are shown. b)(N), without acetamiprid treatment; (T), with acetamiprid treatment (0.08 ppm for the susceptible clones and 0.5 ppm for the resistant clone).Red cells indicate statistically significant differential upregulation (FDR <0.05 and fold change ≥2) between the two clones. The values of FDR and the fold changes of expression levels were calculated using the iDEGES/edgeR method with tag counts (mapped paired-end reads) of two groups of RNA-seq data. The numbers of tag counts (mapped paired-end reads) for each P450 gene are shown for three biological replicates (rep1, rep2, and rep3) of each clone treated with or without acetamiprid. Accession numbers and database sequence identifiers are as follows: c21228_g1 (LC223821), c21368_g1 (LC223822), c29523_g2 (LC223823), c22273_g1 (LC223824), c26291_g2 (LC223825), c26291_g1 (LC223826), c4974_g1 (LC223827), c27644_g1 (LC223828), and c23819_g1 (LC223829).