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. 2018 Oct 2;6:e5703. doi: 10.7717/peerj.5703

Figure 5. Identification and characterization of the miRNA-200-3p binding sites in the 3′ UTR of AjEHHADH and functional effect of miR-200-3p on AjEHHADH.

Figure 5

(A) Schematic representation of the putative miRNA-200-3p targeting sites in AjEHHADH mRNA and the respective mutant sites. (B) HEK-293T cells were co-transfected with the pMIRREPORT-BHMT-WT vector, carrying the wild-type and the mutated AjEHHADH 3′-UTR, pRLCMV-Renilla-luciferase, and control miR-200-3p mimics as indicated. “*”indicates significant differences (P < 0.05). WT, Wild-type; MT, Mutant type; 200M, miR-200-3p mimics; NCM, negative control without miR-200-3p.