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. 2018 Oct 2;6:e5703. doi: 10.7717/peerj.5703

Table 1. Primers used in this study.

Name Primer Sequences (5′–3′) Location
RACE AjEHHADH-R1 GCGAGAATGGCGGAGGGTTTGCAAGT 1,382–1,407
R1-NEST CAGGTATCCCACTGGCGATGAAACA 1,125–1,149
qRT-PCR miR-200-3p-F TAATACTGTCTGGTGATGATG
miR-200-3p-R mRQ 3′ primer
5.8s-F ATCACTCGGCTCGTGCGTC
5.8s-R GCCATTTGCGTTCGAATAAGT
AjEHHADH-F GCTCTTTTCTTCTCTGGCCA 954–973
AjEHHADH-R ACAGGTATCCCACTGCTGAT 1,131–1,150
β- Tubulin-F GAAAGCCTTACGACGGAACA
β- Tubulin-R CACCACGTGGACTCAAAATG
In vivo miR-200-3p mimics-F UAAUACUGUCUGGUGAUGAUG
miR-200-3p mimics-R UCAUCACCAGACAGUAUUAUU
Negative control-F UUCUCCGAACGUGUCACGUTT
Negative control-R ACGUGACACGUUCGGAGAATT
Dual-luciferase AjEHHADH-WT-F CGGATCCGACCCTGTAACAACTCTTAACCTCT 2,025–2,048
AjEHHADH-WT-R CGGATCCGCAACTGACAATTTCTTGTAA 2,280–2,299
AjEHHADH-MUT-F TTCACCAGGTCATAAAACGAGATTTATTTGATC 2,133–2,165
AjEHHADH-MUT-R TAGAGCAATTATGACGACCACTTTAAAGAA 2,126–2,155