Table 1. Primers used in this study.
Name | Primer Sequences (5′–3′) | Location | |
---|---|---|---|
RACE | AjEHHADH-R1 | GCGAGAATGGCGGAGGGTTTGCAAGT | 1,382–1,407 |
R1-NEST | CAGGTATCCCACTGGCGATGAAACA | 1,125–1,149 | |
qRT-PCR | miR-200-3p-F | TAATACTGTCTGGTGATGATG | |
miR-200-3p-R | mRQ 3′ primer | ||
5.8s-F | ATCACTCGGCTCGTGCGTC | ||
5.8s-R | GCCATTTGCGTTCGAATAAGT | ||
AjEHHADH-F | GCTCTTTTCTTCTCTGGCCA | 954–973 | |
AjEHHADH-R | ACAGGTATCCCACTGCTGAT | 1,131–1,150 | |
β- Tubulin-F | GAAAGCCTTACGACGGAACA | ||
β- Tubulin-R | CACCACGTGGACTCAAAATG | ||
In vivo | miR-200-3p mimics-F | UAAUACUGUCUGGUGAUGAUG | |
miR-200-3p mimics-R | UCAUCACCAGACAGUAUUAUU | ||
Negative control-F | UUCUCCGAACGUGUCACGUTT | ||
Negative control-R | ACGUGACACGUUCGGAGAATT | ||
Dual-luciferase | AjEHHADH-WT-F | CGGATCCGACCCTGTAACAACTCTTAACCTCT | 2,025–2,048 |
AjEHHADH-WT-R | CGGATCCGCAACTGACAATTTCTTGTAA | 2,280–2,299 | |
AjEHHADH-MUT-F | TTCACCAGGTCATAAAACGAGATTTATTTGATC | 2,133–2,165 | |
AjEHHADH-MUT-R | TAGAGCAATTATGACGACCACTTTAAAGAA | 2,126–2,155 |