Figure 5. Engineered immunocytokine selectively potentiates the growth of activated adoptively transferred CD8⁺ T cells while boosting immunosuppression in the recipient.

(a) Schematic of the adoptive transfer procedure. CD8⁺ T cells were purified from OT-I/Ly 5.1 mice and adoptively transferred into C57BL/6 mice (Ly 5.2) (n=4 per cohort), which were then stimulated by SIINFEKL peptide and subjected to the indicated treatments for four consecutive days. Mice were sacrificed 48 hours after the final injection and relative expansion was quantified via flow cytometry for the adoptively transferred (AT) CD8⁺ T cells (b) and the recipient T_Reg cells (c), MP CD8⁺ T cells (d), and NK cells (e). Data represent mean ± s.d. Statistical significance was determined by two-tailed unpaired Student’s t-test. The experiment was performed three times with similar results.