Figure 1.

Morpholino knockdown of Kv7.4 in renal and mesenteric arteries. Kv7.4 protein expression in Kv7.4 morpholino transfected mesenteric artery (A, n=5) and renal artery (B, n=4) as normalised to β-actin and expressed as a % of control transfected vessels. Representative blots depicting bands for Kv7.4 and β-actin are displayed in inserts. Concentration dependent contractile responses to U46619 in the mesenteric artery (100pmol/L - 3µmol/L, n=16, C) and to methoxamine in the renal artery (100nmol/L - 30µmol/L, n=10, D) in control (open squares) or Kv7.4 morpholino (closed squares) transfected vessels. Concentration effect relaxations to the Kv7 activator ICA-069673 in the mesenteric artery (100nmol/L - 30µmol/L, n=5, E) or ML213 in the renal artery (30nmol/L - 3µmol/L, n=5, F) in control (open squares) or Kv7.4 morpholino (closed squares) transfected vessels. Concentration effect curve in renal arteries (n=5, G) and mesenteric arteries (n=8, H) to isoproterenol in control (closed squares) and Kv7.4 morpholino (open squares) transfected vessels. Data was analysed by Mann-Whitney test for Western Blots or by Bonferroni post-hoc test following a repeated measures two-way ANOVA for functional studies, where p<0.05 is denoted (*), p<0.01 is denoted (**) and p<0.005 is denoted (***)