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. Author manuscript; available in PMC: 2018 Nov 20.
Published in final edited form as: Free Radic Biol Med. 2018 Feb 2;128:50–58. doi: 10.1016/j.freeradbiomed.2018.01.027

Fig. 5. Basic principle of NOA-1.

Fig. 5

NOA-1 represents a bipartite probe, which consist of the αsGC subunit fused to CGY, a FRET-based cGMP probe, and the βsGC subunit also fused to a CGY (upper panel). Upon NO binding to the βsGC-subunit the active heterodimeric sGC is formed and converts GTP into cGMP (middle panel). The NO-induced formation of cGMP is subsequently visualized with the FRET-based cGMP probe, named CGY (lower panel), which shows increased FRET in the presence of cGMP.