Figure 3. TTX-S Current Density Is Reduced during Torpor.

(A) Exemplar current traces and voltage protocols for activation (left) and steady-state inactivation (right) of TTX-S currents in DRG neurons.

(B) Voltage dependence of TTX-S current. Ordinary two-way ANOVA with Bonferroni correction is shown; ****p < 0.0001 main effect between species. Data are shown as mean ± SEM; n ≥ 17 neurons.

(C) Activation conductance and steady-state inactivation of TTX-S current. Data are shown as mean ± SEM fit with Boltzmann functions. Dashed line indicates average RMP.

(D and E) V₅₀ of activation (D) and steady-state inactivation (E), averaged from individual Boltzmann fits; Mann-Whitney U test. Data are shown as mean ± SEM; n ≥ 16 neurons.

(F) Inactivation rate (tau of decay), calculated from a single-exponential curve fit to the decay of the current at each voltage. Only curves fit with an R² > 0.95 were included. Ordinary two-way ANOVA with Bonferroni correction is shown; p < 0.0001 main effect between species. **p < 0.01; ***p < 0.001; ****p < 0.0001. Data are shown as mean ± SEM; n = 5–18 active; n = 2–17 torpid neurons.

See also Figure S4.