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. 2018 Sep 11;9(71):33482–33499. doi: 10.18632/oncotarget.26060

Figure 2. Cytogenetic analyses of DH-My6 cells.

Figure 2

(A) Giemsa-banding karyotype, showing the following karyotype: 47, XY, ins(1;?)(q21:?), add(3)(p25), der(3)del(3)(p?)t(3;14)(q27;q32), add(8)(q24), -9, add(9)(p11), del(13)(q?), der(14)t(8;14)(q4;q32), add(19)(p13), +mar1, +mar2. (B) FISH analysis with the IGH/MYC dual-color probes, showing fusion signals for the IGH-MYC gene rearrangement (arrows). The green signal corresponds to the normal IGH allele, red to the normal MYC allele, yellow to the IGH-MYC fusion gene, and blue to the centromeric region of chromosome 8. (C) FISH analysis for BCL6 gene rearrangement with the break-apart probes, showing split signals for BCL6 (arrows). Yellow signal corresponds to the intact, nonrearranged BCL6 locus, while separate red (5' BCL6 FISH DNA probe) and green (3' BCL6 FISH DNA probe) signals indicate the BCL6 rearrangement. (D) SKY karyotype (left side, reverse 4′,6-diamidino-2-phenylindole staining; right side, SKY), showing the following karyotype: 47, XY, der(1)(pter→q21::1?::q21→qter), del(3)(p25p26), del(3)(q27), der(8)t(3;8)(q27;q24), der(9)t(9;13)(p13;q14), inv(9)(p21q11), der(12)(pter→q24.1::12?), der(14)t(8;14)(q24;q32), der(19)t(7;19)(?;p13).