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. 2018 Sep 11;9(71):33446–33458. doi: 10.18632/oncotarget.26002

Figure 3. RN765C, a low affinity EGFR ADC, has potent cytotoxicity towards cancers cells and it induces mitotic arrest and cell death.

Figure 3

Normal human epidermal keratinocytes (A), NSCLC cell line HCC827 (B), pancreatic cancer cell line BxPC3 (C) and EGFR negative colorectal cancer cell line SW620 (D), were exposed to the indicated agents over a range of concentrations for 4-5 days. Relative viable cell number was determined at the end of treatment. Free payload and negative control ADC (neg ctrl ADC) generated with the same conjugation site and linker payload were included as references. Unconjugated EGFR antibodies: cetuximab, EGFR.Ab-L (low affinity) and EGFR.Ab-H (high affinity). (E) and (F), MDA.MB.468 cells were exposed to 2 µg/mL RN765C, 2 µg/mL neg ctrl ADC or 5 nM free-payload PF-06380101 for the indicated duration. At the end of treatment, cells were harvested and DNA content was measured to determine the % of G2/M phase cells (E) and % of apoptotic and dead cells (F).