Table 1. Binding kinetics of EGFR affinity variant antibodies to human and cynomolgus EGFR proteins at 37oC.
| EGFR antibodies | human EGFR | cyno EGFR | ||||||
|---|---|---|---|---|---|---|---|---|
| ka (1/Ms) | kd (1/s) | KD (nM) | t1/2 (s) | ka (1/Ms) | kd (1/s) | KD (nM) | t1/2 (s) | |
| EGFR.Ab-H | 6.29E+05 | 1.01E-02 | 16.1 | 68.6 | 6.10E+05 | 8.65E-03 | 14.2 | 80.1 |
| EGFR.Ab-M | 5.35E+05 | 1.05E-01 | 196 | 6.60 | 4.90E+05 | 8.77E-02 | 179 | 7.90 |
| EGFR.Ab-L | 6.21E+05 | 2.10E-01 | 338 | 3.30 | 5.50E+05 | 1.87E-01 | 341 | 3.71 |
Affinity of EGFR antibodies to recombinant monomeric 8xHis-tagged extracellular domain of human EGFR and cynomolgus EGFR protein was determined by surface plasmon resonance.