| Reporter and other plasmids |
| pBS-(CBR-TCR(PTC))-(CBG-TCR(WT)) |
Generated in lab |
N/A |
| pBS-(CBR-TCR(PTC)) |
Generated in lab |
N/A |
| pBS-(CBG-TCR(WT)) |
Generated in lab |
N/A |
| pAdenoX-PRLS-ZsGreen1 |
Clontech |
Cat#632258 |
| pLenti-Cas9 Blast |
Addgene |
52962 |
| pCMV-VSVG |
Addgene |
8454 |
| pPAX2 |
Addgene |
12260 |
| plentiGuide-Puro |
Addgene |
52963 |
| His-hSMG1 (WT) |
Dr.Shigeo Ohno |
N/A |
| His-hSMG1 (DA) |
Dr.Shigeo Ohno |
N/A |
| GST-p53 |
Dr. Robert T. Abraham |
N/A |
| siRNAs/gRNAs |
| siAMPK α1 |
Ambion |
GAAGATCGGCCACTACATTC |
| siAMPK α2 |
Ambion |
GAAGATCGGACACTACGTGC |
| siATF4#1 |
Ambion |
S1702 |
| siATF4#2 |
Ambion |
S1703 |
| siControl |
Ambion |
Cat#4390846 |
| sgAMPK α1 |
IDT |
GAAGATCGGCCACTACATTC |
| sgAMPK α2 |
IDT |
GAAGATCGGACACTACGTGC |
| sgATF4 |
IDT |
AACCTCTTCCCCTTTCCCCA |
| Antibodies |
| Mouse anti-HA antibody |
Biolegend |
Cat#901507 |
| Mouse anti-β-Actin |
Thermo Fisher Scientific |
Cat#MA5-15739 |
| Rabbit anti-AMPK α |
Cell Signaling Technology |
Cat#2532 |
| Rabbit anti pACC1 Ser79 |
Cell Signaling Technology |
Cat#11818 |
| Rabbit anti-ACC1 |
Cell Signaling Technology |
Cat#3676 |
| Rabbit anti-SMG1 |
Cell Signaling Technology |
Cat#9149 |
| Rabbit anti-p53-pS15 |
Cell Signaling Technology |
Cat#9284 |
| Rabbit anti-GST |
Raised against GST-GFP |
N/A |
| Rabbit anti-UPF1 |
Cell Signaling Technology |
Cat#12040 |
| Rabbit anti-SMG6 |
Abcam |
Cat#AB87539 |
| Rabbit anti-UPF2 |
Novus Biologicals |
Cat#NB2-20813 |
| Rabbit anti-UPF3B |
Bethyl laboratories |
Cat#A303-688A |
| Rabbit anti-SMG5 |
Abcam |
Cat#AB129107
|
| Rabbit anti-SMG7 |
Bethyl Laboratories |
Cat#A302-170A |
| Rabbit anti-LC3B |
Cell Signaling Technology |
Cat#2775 |
| Rabbit anti-ATF4 |
Cell Signaling Technology |
Cat#11815 |
| Alexa Fluor 488-conjugated goat anti-rabbit |
Thermo Fisher Scientific |
Cat#A-11008 |
| Chemicals |
| D-Luciferin |
Sigma |
Cat#L9504 |
| Actinomycin D |
Sigma |
Cat#A1410 |
| Caffeine |
Sigma |
Cat#C0750 |
| Compound C (CC) |
Sigma |
Cat#5499 |
| Thapsigargin |
Sigma |
Cat#T9033 |
| AICAR |
TOCRIS |
Cat#2840 |
