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. 2018 Oct 5;13(10):e0205036. doi: 10.1371/journal.pone.0205036

Fig 7. AmgRS-independent macrolide induction of mexAB-oprM expression.

Fig 7

(A) mexA expression was assessed in log-phase cultures of WT P. aeruginosa strain K767 exposed to the MIC of tetracycline (TET; 16 μg/ml), erythromycin (ERY; 512 μg/ml) and azithromycin (AZI; 512 μg/ml) for 30 min using qRT-PCR. Expression was normalized to rpoD and is reported to that of the untreated (-) strain K767. (B) mexA expression was assessed in log-phase cultures of P. aeruginosa strains K767 (AmgR+) and K3159 (AmgR-) exposed to the MIC of ERY or AZI (512 μg/ml for both drugs and both strains) for 30 min using qRT-PCR. In all cases, expression was normalized to rpoD and is reported relative to that of the untreated strain K767 (fold change). Values are means ± SEMs from at least three independent determinations, each performed in triplicate. t-test: **, P < 0.01; ***, P < 0.001.