Table 3. Knockout of five transcriptional regulators (TR) and two core microcolony (MC) genes result in defects in microcolony formation and function.
C. albicans deletion mutants were grown on oral epithelial cells (OEC); and microcolony formation, adhesion and invasion were measured. Significant (p < 0.05) differences in microcolony density, number of adhered cells, or percentage of cells actively invading were determined using one-way ANOVA followed by a post-hoc Tukey’s t-test (microcolony formation) or Bonferroni (adhesion and invasion). Strains highlighted in grey were defective in all three categories. ND: not determined.
| Microcolony Formation |
OEC Adhesion |
OEC Invasion | |
|---|---|---|---|
| C. albicans TR | |||
| Δrob1 | Defective | Defective | Defective |
| Δndt80 | Defective | Defective | Defective |
| Δsfl1 | Increased | Defective | Increased |
| Δsfl2 | Defective | Defective | Defective |
| Δmcm1 | Defective | Defective | Defective |
| Δtye7 | Defective | No defect | No defect |
| Δrca1 | Defective | No defect | No defect |
| Δnrg1 | Defective | ND | Defective |
| Core MC Genes | |||
| Δhwp1 | Defective | Defective | Defective |
| Δece1 | Defective | No defect | No defect |
| Δihd1 | No Defect | No defect | No defect |
| Δhyr1 | Defective | No defect | No defect |
| Δpga10 | Defective | Defective | Defective |
| ΔC6_03600C_A | No Defect | No Defect | No Defect |
| Δsap5 | No Defect | Defective | Defective |
| Δlip8 | No Defect | No defect | No defect |