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. 2018 Sep 25;14(9):e1007315. doi: 10.1371/journal.ppat.1007315

Fig 8. In vitro splicing assay.

Fig 8

Permeablised procyclic-form trypanosomes (2.5 x 108 / reaction) were incubated for 2 min in the presence of DMSO or AN7973, and then for 10 min in the presence of alpha-32P-UTP. Newly-made RNA was analysed by denaturing gel electrophoresis and autoradiography. The full-length SLRNA (~140nt) and the de-branched SLRNA intron (~100 nt) are indicated.