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. 2018 Sep 25;14(9):e1007315. doi: 10.1371/journal.ppat.1007315

Fig 9. Effects of benzoxaborole treatment on formation of YFP-DHH1 granules.

Fig 9

A-C) Bloodstream-form trypanosomes with one DHH1 gene tagged in situ were washed with PBS, fixed with formaldehyde, then allowed to adhere to glass slides before imaging to detect YFP-tagged DHH1 (magenta) and DNA (cyan). Cells were (A) untreated, (B) incubated with Sinefungin (1μg/mL 30 min), or (C) treated with AN7973 (230 nM, 2h). D) Results for all tested oxaboroles. The average percentages of cells with clear peri-nuclear granules are plotted on the y-axis and the % Y-structure is on the x-axis. Times to show an effect on growth are as in Fig 6B. Results for the three replicate experiments are in S5 Fig and typical fields are shown in S6 and S7 Figs.