Table 1. Proteotypic peptides of EGFR, HER2, HER3, PTEN and phospho-HER2, their m/z at defined charge, the range of light and heavy peptides injected for calibration curve.
| PROTEIN | PEPTIDE | m/z | charge | range light | heavy | LLOD | LLOQ | CV (%) | SE |
|---|---|---|---|---|---|---|---|---|---|
| HER2 | GTPTAENPEYLGLDVPV | 884.4411 | 2 | 1−100 fmol | 10 fmol | <1 fmol | 10 fmol | 2.8 | 0.019 |
| GLQSLPTHDPSPLQR | 823.4365 | 2 | 1−100 fmol | 10 fmol | <1 fmol | 5 fmol | 7.0 | 0.112 | |
| 549.2934 | 3 | 1−100 fmol | 10 fmol | <1 fmol | 5 fmol | 3.1 | 0.045 | ||
| GIWIPDGENVK | 614.322 | 2 | 1−100 fmol | 10 fmol | <1 fmol | 10 fmol | 2.7 | 0.014 | |
| SGGGDLTLGLEPSEEEAPR | 957.458 | 2 | 1−100 fmol | 10 fmol | <1 fmol | 5 fmol | 2.6 | 0.009 | |
| phospho HER2 | GLQSLPTHDPSPLQR | 863.4196 | 2 | 1−100 fmol | 20 fmol | 2 fmol | 5 fmol | 8.5 | 0.007 |
| 575.9488 | 3 | 1−100 fmol | 20 fmol | <1 fmol | 5 fmol | 12.0 | 0.009 | ||
| GTPTAENPEYLGLDVPV | 926.4242 | 2 | 1−100 fmol | 20 fmol | 2 fmol | 2 fmol | 8.4 | 0.002 | |
| EGFR | GSTAENAEYLR | 605.7886 | 2 | 1−100 fmol | 10 fmol | <1 fmol | 2 fmol | 2.0 | 0.004 |
| IPLENLQIIR | 604.8717 | 2 | 1−100 fmol | 10 fmol | 2 fmol | 10 fmol | 9.3 | 0.026 | |
| HER3 | GVWIPEGESIK | 607.8244 | 2 | na | na | na | na | na | na |
| LAEVPDLLEK | 563.8213 | 2 | na | na | na | na | na | na | |
| PTEN | YFSPNFK | 451.724 | 2 | 1−100 fmol | 10 fmol | <1 fmol | 2 fmol | 6.8 | 0.015 |
| GVTIPSQR | 429.2456 | 2 | 1−100 fmol | 10 fmol | <1 fmol | 2 fmol | 1.8 | 0.003 |
Determination of lower limit of detection (LLOD), lower limit of quantification (LLOQ) Coefficient of Variation (CV) in percent and Standard-error (SE).