Calculated binding modes of the protein-ligand
complexes: (a) GFRα1–BT13 at the GDNF–GFRα1
interface, region A (ΔGbind = −6.0
kcal/mol); (b) GFRα1-BT18 at the GDNF–GFRα1 interface,
region A (ΔGbind = −6.4 kcal/mol);
(c) RetA–BT13 at the RetA–GFRα1 interface, region
B (ΔGbind = −6.7 kcal/mol);
(d) RetA-BT18 at the RetA–GFRα1 interface, region B (ΔGbind = −7.5 kcal/mol); (e) GFRα1–BT13
at the allosteric site, region C (ΔGbind = −7.9 kcal/mol); and (f) GFRα1–BT18 at the
allosteric site, region C (ΔGbind = −9.8 kcal/mol). The amino acid residues of protein (PDB
code: 4UX8)
are colored as gray (carbon), blue (nitrogen), red (oxygen), and white
(hydrogen). Intermolecular hydrogen bonds are shown by dashed lines.