Table 2. Transfection Efficacy of Formulations [5b(chol) and 5d(chol)] of the Two GAs Containing Cholesterol and DOPE as Helper Lipids in the Absence/Presence of Serum in the Two Cell Lines.
| transfection
efficacy in cell lines |
|||||||
|---|---|---|---|---|---|---|---|
| in absence of serum |
in presence of serum (10% FBS) |
||||||
| GA used | formulation | molar ratios of GA/DOPE/cholesterol | best N/P ratio | A549 | HeLa | A549 | HeLa |
| 5b | 5b(chol) | 1:1:1 | 1 | 1.96 | 2.95 | 1.44 | 2.45 |
| 1:1:0.5 | 1 | 1.81 | 2.74 | 1.36 | 2.38 | ||
| 5d | 5d(chol) | 1:1:1 | 2 | 1.94 | 3.61 | 1.72 | 3.39 |
| 1:1:0.5 | 2 | 1.73 | 3.07 | 1.68 | 2.92 | ||