Figure 3. The function of condensin is dispensable for gene regulation during S and G2 phases of the cell cycle in fission yeast.

(A–C) Gene expression was assessed in synchronized cut14-208 cells progressing from early S phase to G1 phase at the restrictive temperature. (A) Scheme of the experiment.(B) Left panel: FACScan analyses. Right panels: chromosome segregation and cytokinesis assessed by staining DNA with DAPI and the septum with calcofluor (n >= 100). (C) Total RNA extracted from cdc10-129 and cdc10-129 cut14-208 cells shown in (B), was reverse-transcribed in the presence or absence of RT and cDNA quantified by qPCR. Red line = 1. Shown are averages ± SDs measured from biological triplicates. (D–F) Gene expression was assessed in synchronized cut14-208 cells progressing from early S phase to late G2 phase at the restrictive temperature. (D) Scheme of the experiment. (E). FACScan analyses. (F) Total RNA extracted from cdc25-22 and cdc25-22 cut14-208 cells shown in (E) was reverse-transcribed in the presence or absence of RT and cDNA quantified by qPCR. Shown are averages ± SDs measured from biological triplicates. (G–I) Gene expression was assessed in synchronized cut14-208 cells progressing from early S phase to metaphase at the restrictive temperature. (G) Scheme of the experiment. Thiamine repressed the nmt41-slp1 gene in order to arrest cells in metaphase. (H) Percentages of mononucleate, mitotic cells from n = 3 experiments. (I) Total RNA extracted from nmt41-slp1 and nmt41-slp1 cut14-208 cells shown in (H) was reverse-transcribed in the presence or absence of RT and cDNA quantified by qPCR. Shown are averages ± SDs measured from biological triplicates.