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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Thromb Haemost. 2018 Aug 16;16(10):2097–2105. doi: 10.1111/jth.14251

Figure 3.

Figure 3

Cooperation of the N- and C- AIM is required for its inhibitory effect. (A) Illustration of rVWF1238- 1461 that misses the C-AIM. (B) Comparing the rVWF1271-1493 and rVWF1238-1461 by a coomassie blue- stained polyacrylamide gel under nonreducing conditions. (C) Comparing the binding to immobilized GPIb- IX among rVWF1261-1472, rVWF1271-1493, rVWF1238-1461, and rVWF1238-1493 by ELISA (n = 3). (D) Representative histogram of flow cytometry measurement to determine the binding of rVWF1261-1472, rVWF1271-1493, and rVWF1238-1461 to platelets in platelet rich plasma. (E) Whisker plot of the melting temperatures of A1 fragments. Mean value ± standard deviation (n=4) was marked under each plot. A paired t-test was conducted using GraphPad Prism 5 (GraphPad Software, Inc. CA the USA) and the pvalue was marked.