Skip to main content
. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Hepatology. 2018 Oct;68(4):1519–1533. doi: 10.1002/hep.29915

Fig. 5. Serum levels of arginine metabolism-related amino acids in bile duct-ligated mice.

Fig. 5

(A) Serum levels of arginine, ornithine, citrulline, aspartate, glutamate, and glutamine in sham operated mice (Sham) or in mice subjected to BDL and sacrificed on postoperative day 14 (BDL Day 14). (B) Renal and hepatic expression of nitric oxide synthase isoforms: Nos1–neuronal, Nos2–inducible, Nos3–endothelial, in sham operated mice (Sham–black bars) or in mice subjected to bile duct-ligation and sacrificed on postoperative day 7 (BDL Day 7–blue bars) or day 14 (BDL Day 14–red bars), respectively. Enzyme activity of arginases (C), and endothelial nitric oxide synthase (D). (E) Renal levels of cyclic guanosine monophosphate (cGMP) in sham operated mice (Sham–black bars) or in mice subjected to bile duct-ligation and sacrificed on postoperative day 14 (BDL Day 14–red bars). (F) 3-nitrotyrosine (3-NT) expression analysis, as a marker of nitrative stress by immunostaining in sham operated mice (Sham) or in mice subjected to BDL and sacrificed on postoperative day 14 (BDL Day 14). Scale bar represent 100 μm. Results are mean±S.E.M. *p<0.05 vs. sham, n=10–15 for amino acid analysis, n=6–8 in other experiments.