Figure 10. Sox2 regulates the rate of oligodendrocyte production – evidences from Plp-CreER^T2:Sox2^fl/fl transgenic mice.

A, transgenic mice and experimental designs for Panels B–F. Plp-CreER^T2:Sox2^fl/fl (Sox2 cKO) and non-Cre Ctrl mice were injected intraperitoneally with tamoxifen at P10, P11 and P13 once a day, and sacrificed 24 h after the last injection. B, representative confocal images showing Sox2 is expressed in Olig2⁺ oligodendroglial lineage cells in non-Cre Ctrl (upper panels, arrowheads) and absent from most Olig2⁺ cells in P14 spinal cord of Sox2 cKO mice (lower panels, arrowheads). Scale bars = 10 μm. C, densities (per mm²) of Olig2⁺PDGFRα⁺ OPCs. Two-tailed Student’s t test [t₍₇₎ = 0.972 WM, t₍₇₎ = 0.661 WM+GM]. D, densities (mm²) of Olig2⁺CC1⁺ OPCs. Two-tailed Student’s t test [t₍₇₎ = 2.088 WM, t₍₇₎ = 2.258 WM+GM]. E, representative confocal images showing reduction of APC⁺TCF7l2⁺ premyelinating OLs in P14 spinal cord. Scar bars = 10 μm. F, density (per mm²) of APC⁺TCF7l2⁺ cells. Two-tailed Student’s t test [t₍₇₎ = 3.163 WM, t₍₇₎ = 3.528 WM+GM].