Table 1.
KIR2DL PCR primers and target site positions.
| Reaction | Primers name | Nucleotide targeted | Sequence Primers | Size amplicon (bp) |
|---|---|---|---|---|
| Control | ControlF | NA | CCAAGCCCAACCTTAAGAAGAAAATTGGAG | 813 |
| ControlR | NA | CCAAACCCACGGTACGCATGGGAACACTGC | ||
| 2DL1 Reaction 1 | 2DL1R1F | 3680 | AGAGATAAGACACCAGGAAGGGGAAGCCCG | 388 |
| 2DL1R1R | 4011 | TGTCCAGAGGGTCACTGGGAGCTGACTC | ||
| 2DL1 Reaction 2 | 2DL1R2F | 5499 | GAGAGAGAGAGAGAGAGAGCATTAGGTCATAGTA | 383 |
| 2DL1R2R | 5820 | TGACTTTGACCACTCGTATGGAGAGTCTT | ||
| 2DL1 Reaction 3 | 2DL1R3F | 13420 | ATCCTCTTCATCCTCCTCTTCTTTCTCCTTCACT | 252 |
| 2DL1R3R | 13609 | CAGTTCAGAATCAGGCAACGGTCTGTGAAT | ||
| 2DL1 Reaction 4 | 2DL1R4F | 5499 | GAGAGAGAGAGAGAGAGAGCATTAGGTCATAGGA | 297 |
| 2DL1R4R | 5735 | TGGCCTGGAATGTTCCGTTGACCTTGCT | ||
| 2DL1 Reaction 5 | 2DL1R5F | 3790 | AACCTTCCCTCCTGGCCCACCCAGGTAC | 278 |
| 2DL1R5R | 4011 | GATGTCCAGAGGGTCACTGGGAGCTGACGC | ||
| 2DL1 Reaction 6 | 2DL1R6F | 5616 | ATATGAGAAACCTTCTCTCTCAGCCCAGTT | 202 |
| 2DL1R6R | 5761 | GTGGGTGGCAGGGCCCAGAGGAAAGTAA | ||
| 2DL1 Reaction 7 | 3DP1F | NA | ACGTGTTGTGAGTTGGTCATAGTGA | 649 |
| 3DP1VF | NA | AAGTGGAAATGGGAGAATCTTCTGAC | 382 | |
| 3DP1R | NA | GCCCTCTGACCTGTGACCATGATC | ||
| 2DL1 Optional 1 | 2DL1O1F | 71 | GTTGGTCATAGTGAAGGACACTAGGTGTCAAATTCTATC | 274 |
| 2DL1O1R | 281 | TCACCAACACACGCCATGCTGACGTC | ||
| 2DL1 Optional 2 | 2DL1O2F | 281 | CTCCGGCAGCACCATGTCGCTCTTAT | 390 |
| 2DL1O2R | 620 | CCGTAACTCCACCTCCAGGCCCATTA | ||
| 2DL1 Optional 3 | 2DL1O3F | 3787 | AAACCTTCCCTCCTGGCCCACCCAAA | 376 |
| 2DL1O3R | 4110 | CTTCCTTACAGCCACCTGGGTCTCCAGT | ||
| 2DL1 Optional 4 | 2DL1O4F | 3942 | GGGTCTCCAAGGCCAACTTCTCCATGG | 222 |
| 2DL1O4R | 4110 | CTTCCTTACAGCCACCTGGGTCTCCACT | ||
| 2DL2 Reaction 1 | 2DL2R1F | 5663 | TATCCAGGGAGGGGGAGGCCCATGATT | 211 |
| 2DL2R1R | 5820 | TGAGACAGATATGGGGTTTCCTCACCAG | ||
| 2DL2 Reaction 2 | 2DL2R2F | 5663 | TATCCAGGGAGGGGGAGGCCCATGATT | 210 |
| 2DL2R2R | 5820 | GAGACAGATATGGGGTTTCCTCACCCA | ||
| 2DL2 Reaction 3 | 2DL2R3F | 13995 | ACAGATGCTGCGGTAATGGACCAAGATT | 309 |
| 2DL2R3R | 14249 | ATCTGGACTCAGCATTTGGAAGTTCCCC | ||
| 2DL2 Reaction 4 | 2DL2R4F | 11984 | CTACTTCCAATCACCTGTGGAGATTCATG | 2322 |
| 2DL2R4R | 14249 | ATCTGGACTCAGCATTTGGAAGTTCCTT | ||
| 2DL2 Optional 1 | 2DL2O1F | 3754 | AACCTTCCCTCCTGGCCCACCCAGGTTC | 191 |
| 2DL2O1R | 3890 | CATCATGGGACCGATGGAGAAGTTGGTT | ||
| 2DL2 Optional 2 | 2DL2O2F | 3754 | AACCTTCCCTCCTGGCCCACCCAGGTAG | 191 |
| 2DL2O2R | 3890 | CATCATGGGACCGATGGAGAAGTTGGGT | ||
| 2DL3 Reaction 1 | 2DL3R1F | 13892 | ATGAAATGAGGGCCCAGAAGTGCCCTGT | 314 |
| 2DL3R1R | 14154 | GGTGTCTTGGGCCTCTGAGAAGGAC | ||
| 2DL3 Reaction 2 | 2DL3R2F | 3825 | CACAGAGAAGGGAAGTTTAAGGACACTTTGTG | 399 |
| 2DL3R2R | 4168 | TGTATGGCCCCTGTGTCTGTCCTTT | ||
| 2DL3 Reaction 3 | 2DL3R3F | 9063 | CTGTCTCATGTTCTAGGAAACCCTTCAAATAGTTGGGT | 319 |
| 2DL3R3R | 9303 | GAAGGATGTCAGATTGGCAATCATTCTTCTAGCTTGTAGGAAA | ||
| 2DL3 Reaction 4 | 2DL3R4F | 13973 | GCCTGCAGGGAACAGAACAGTGAACAAG | 233 |
| 2DL3R4R | 14154 | GGTGTCTTGGGCCTCTGAGAAGGCT | ||
| 2DL3 Reaction 5 | 2DL3R5F | 3853 | CCTCATTGGAGAGCACCATGATGGGGCT | 430 |
| 2DL3R5R | 4222 | CCTCTCTCTGGGACATGTCTGTCTGTCTGTCTGT | ||
| 2DL3 Optional 1 | 2DL3O1F | 3708 | TAGGAGTCCACAGAAAACCTTCCCTCGG | 323 |
| 2DL3O1R | 3976 | GAATGTCCGGACACTCTCACCTGTGACG | ||
| 2DL3 Optional 2 | 2DL3O2F | 16795 | CCCTCCATCTGGGTGCTTGTCCTAAAGGCG | 213 |
| 2DL3O2R | 16949 | GCGATGAAGGAGAAAGAAGAGGAGGAGGTC | ||
| 2DL3 Optional 3 | 2DL3O3F | 17646 | TGAACAAGACCCTCAGGAGGTGACATTT | 169 |
| 2DL3O3R | 17761 | TCATGGGCAGGAGACAACTTTGGATAT | ||
| 2DL3 Optional 4 | 2DL3O4F | 7315 | TCCTGCAATGTTGGTCAGATGTCAGGTTCG | 643 |
| 2DL3O4R | 7903 | AGGCCACAGGGCCCAACTCAGGTCGT | ||
| 2DL3 Optional 5 | 2DL3O5F | 13892 | ATGAAATGAGGGCCCAGAAGTGCCCTGT | 278 |
| 2DL3O5R | 14111 | CTCTGTGTGAAAACGCAGTGATTCAACTGTTT | ||
| 2DL3 Optional 6 | 2DL3O6F | 13892 | ATGAAATGAGGGCCCAGAAGTGCCCTGT | 278 |
| 2DL3O6R | 14111 | CTCTGTGTGAAAACGCAGTGATTCAACTGTTC |