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. 2018 Oct 5;8:14888. doi: 10.1038/s41598-018-33134-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of emitted fluorescence for the different groups. (a–c) Topological representation of the emitted fluorescence spectra at different excitation wavelengths for the control (a,d,g), the metastasis (b,e,h) and the GBM (c,f,i) groups. (d–f) Represent colormaps of the Emission-Excitation matrix for each type of tissue. The ideal excitation wavelength is highlighted by a red line and the part of the map corresponding to the SHG signal is identified by a green arrow. TPEF images (g–i) of the selected region is also shown with a scale bar of 100 microns.