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. 2018 Oct 5;8:14888. doi: 10.1038/s41598-018-33134-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spectral decomposition and comparison of emitted spectra at 890 nm for each group. (a–c) Example of fitted spectra for control (a), metastasis (b), and GBM (c), under 890 nm excitation. (d) Mean spectra and standard deviations determined from 25 fresh human samples (10 metastasis samples, 8 GBM samples and 7 control samples) along with representative images of TPEF (red) and SHG (green) corresponding to each tissue group.