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. 2018 Oct 5;8:14872. doi: 10.1038/s41598-018-33293-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of SGLT1 and SGLT2 in hiPSC-derived cardiomyocytes and human heart tissue. (A) Confirmation of the identity of the PCR product obtained from SGLT2-specific amplifications by DNA sequencing analysis. (B,C) Expression of SGLT1 and SGLT2 in human heart tissue were confirmed by PCR analysis and DNA sequencing analysis. (D) Protein levels of SGLT1 and SGLT2 in the hiPSC-derived cardiomyocytes were evaluated by Western blot analysis. *p < 0.05. For the full-length images of gels and blots shown in this figure, please refer to Supplementary Figs S4 and S5.