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. 2018 Oct 5;8:14890. doi: 10.1038/s41598-018-33232-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of T-cell populations. Female BALB/c mice were subjected to femur fracture, splenectomy and hemorrhagic shock (TSH) or femur fracture and hemorrhagic shock (TH). Absolute numbers of circulating (a). CD4+CD25+CD127− T-cells, (b). CD8+ T-cells and (c). CD4+ T-cells at BL (induction of trauma), 24 h and 48 h time point after TSH or TH. For TH: at BL n = 24, 24 h n = 16, 48 h n = 12. For TSH: BL n = 11, 24 h n = 6, 48 h n = 5. *p < 0.05.