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. 2018 Oct 5;8:14864. doi: 10.1038/s41598-018-33115-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Amber codon suppression at site Leu54 in the ACP-GFP fusion construct screened in reporter assay. (A) Overview of ncAAs used in this study. (B) Best expression efficiency of different ncAAs (left panel) and comparison of some representatives of the screening (right panel). Respective plasmids used for incorporation of ncAAs are indicated by plasmid number (#; listed in Supplementary Table S2). A compilation of all results from the reporter assay can be found in Supplementary Fig. S2. Expression efficiency is read out by GFP fluorescence of 2 mL E. coli cell cultures and compared to wild type reference (taken as 100%). For incorporation, 2 mM ncAAs were supplemented to the medium. Cultures lacking ncAAs were taken as negative control to determine background signal. Dots refer to values of the biological samples. The averages of biological samples are plotted together with standard deviations. Technical errors were below 10%.