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. 2018 Oct 5;8:14893. doi: 10.1038/s41598-018-33180-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of cellular composition, morphology and polarisation of differentiated BBECs and ex vivo bronchial epithelium using fluorescent immunohistochemistry. Ex vivo bronchial epithelium dissected from the donor animal before cell extraction (i) and day 21 post-ALI BBEC cultures (ii) were fixed and paraffin-embedded using standard histological techniques. Sections were cut, deparaffinised and immunohistochemically stained to identify markers of specific epithelial cell-type as follows: cilia (ciliated cells) - red; Muc5AC (mucus and goblet cells) - green; p63 (basal cells) - blue; nuclei - grey.