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. 2018 Oct 5;8:14889. doi: 10.1038/s41598-018-33245-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The light microscope images of the cross-sections of conventional (A) and dual network (B) scaffolds prepared and stained using standard histology/tissue processing protocols. The diffusion of fluorescein sodium salt as the model substance into the conventional and dual network scaffolds after 0 (C1), 0.5 (C2) and 1.5 (C3) h immersion in 10 µg/ml aqueous solution. Micro-CT 3D image of the dual network scaffold (D). Pore size distribution profile of the dual network scaffold (E1), % total pore volume and cumulative surface area as a function of pore size (E2) as well as pore volume, surface area and cumulative surface area for different ranges of pore size (E3) obtained by mercury intrusion porosimetry. The SEM images of primary pores (F1), porous wall of a primary pore (F2) and the opening of the secondary pores (F3) of the dual network scaffold.