Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 5;23:49. doi: 10.1186/s11658-018-0113-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 3 — Identification of mesenchymal tissue stem cells (MSCs) in the cartilage proliferation model. CD44 and CD90 are markers for MSCs of auricular cartilage and Ki-67 protein is a cellular marker for proliferation. a, d, g: Biochemical evaluation of rabbit perichondrium tissue immunostained with anti-CD90 peroxidase-conjugated antibody; b, e, h: biochemical evaluation of rabbit perichondrium tissue immunostained with anti-CD44 peroxidase-conjugated antibody; c, f, i: biochemical evaluation of rabbit perichondrium tissue immunostained with anti-Ki67 peroxidase-conjugated antibody. a-c: Pre-injection of bFGF; d-f: week 1 post bFGF-injection; g-i: week 2 post bFGF-injection. A small number of CD44-positive and CD90-positive cells was observed in the noninjected group (a, b), but a large number of CD44-positive and CD90-positive cells was observed 1 week after the injection of bFGF. Most of spindle- and spider-shaped cells in the perichondrium layer stained positive for CD44 and CD90 (d, e). These positive cells decreased in number as the perichondrium layer thinned 2 weeks after the injection (g, h). Ki-67-positive cells were scarcely observed in noninjected controls or one day after injection (c). During week 1, Ki67-positive perichondrial cells were observed in the outer layer from the perichondrial inner-layer/outer-layer boundary (f). Only perichondrial cells stained positive for the Ki-67 marker in the superficial layer of the perichondrium during week 2 (i). “Reprinted with permission from (Yagishita, M. 2013. Involvement of mesenchymal tissue stem cells and aquaporin 1 in rabbit auricular cartilage regeneration from perichondrium with full reference. Journal of Kanazawa Medical University, 38, 43–52.). Copyright (2013) publication administration of Kanazawa Medical University”