Fig. 5.

Distribution of TrxG components in erythroid cells. a The α-globin locus, with shaded areas indicating CGIs (green) and putative regulatory regions (blue). ChIP signal names indicated to left. Pileups are shown scaled to 1x genome coverage, with full scale 0–50 × depth. b Heatmaps showing normalised/input-subtracted erythroid signals for CFP1 and other TrxG subunits. ChIP indicated directly above each heatmap, and TrxG complex indicated above that. Upper, TSSs; middle, putative enhancers; and lower, CGIs (ordered by CGI length). c Colocalisation of subunits of SET1A methyltransferase complexes in a high-confidence peak-set. Light blue and light red regions represent regions with CFP1 and SET1A peaks, respectively. Red outline represents colocalisation between CFP1 and SET1A. Blue outline represents CFP1 peaks colocalised with RBBP5. Number in bold represents overlap of all four SET1A complex subunits (CFP1, SET1A, HCF1, and RBBP5). d Specialisation of TrxG complexes for enhancers and TSSs. “Other” regions are regions containing peaks of at least two TrxG complex subunits that colocalised neither to TSSs nor putative enhancers defined earlier in the study. SET1A/B complexes are represented by CFP1/SET1A, MLL1/2 complexes by MENIN and MLL3/4 by UTX. Note: error bars are derived by assuming that similarly prepared ChIP experiments give a number of peaks governed by Poisson statistics