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. 2018 Oct 6;11:274. doi: 10.1186/s13068-018-1279-5

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Experimental design. Lab-scale biogas reactors were fed with VFAs using two different feeding regimes (R_disco and R_conti), two different HRTs and two different substrate concentrations. The microbial community was analyzed based on the 16S rRNA gene (amplicon sequencing) for bacteria and the mcrA gene (T-RFLP) for methanogenic archaea. Substrate concentration of 37.2 gCOD L⁻¹ corresponds to 27.4 g L⁻¹ and 0.54 M