Fig. 3.

Effect of 17β-estradiol, ATP and tamoxifen on IB3–1 single cell membrane current density. a current/voltage relationship of control cells (squares; n = 12) and cells in the presence of 300 μM NPPB, a chloride channel blocker (triangles; n = 6). b current density versus membrane voltages in control cells (squares; n = 12), in the presence of 10 nM 17β-estradiol (triangles; n = 5) or in the presence of 100 μM ATP (circles; n = 4). ATP was not able to increase current density once in combination with 10 nM 17β-estradiol (diamonds; n = 5). c current/voltage relationship of control cells (squares; n = 12), after perfusion with 10 μM TMX (circles; n = 6) in the presence of 10 nM 17β-estradiol. The addition of 100 μM ATP induced a marked increase in current density (triangles; n = 5). d and e current density box chart plots at + 80 mV membrane voltage related to the experiments reported in B and C