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. 2018 Oct 5;18:62. doi: 10.1186/s12896-018-0473-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Combinatorial optimization analysis of Mxe GyrA intein cleavage activity in CeA–Mxe–ELK16 fusion. Different incubation times (a) and concentrations of DTT (40–80 mM) (b) were used to optimize cleavage activity. Different incubation temperatures (4 °C or 25 °C) were also tested (c). P, samples before cleavage with DTT; M, protein marker