Figure 3.

CdiA‐CT^STECO31 is a specific tRNase. Northern blot analyses of RNA isolated from intoxicated E. coli cells. The C‐terminal toxin domains of Rhs1 from E. cloacae ATCC 13047 (ECL Rhs1: YP_003612075.1), CdiA from K. pneumoniae 342 (Kpn 342: ACI08381.1), CdiA from E. coli STEC_O31 (STEC O31: EJK94116.1) and CdiA E. coli 3006 (EC3006: EKI34460.1) were expressed in E. coli, and total RNA was isolated for Northern blot hybridization using radiolabeled probes to the indicated tRNAs. Anticodon sequences are shown in parentheses for specific isoacceptors. Arrows to the left of the blot indicate cleavage products detected in CdiA‐CT^STECO31 intoxicated cells. [Colour figure can be viewed at http://wileyonlinelibrary.com]