Figure 7.

Diversification of prokaryotic EndoU toxin domains. A. EndoU domains from predicted CdiA proteins were aligned using Clustal‐Omega, and the associated unrooted tree rendered using the Tree of Life website. The source multiple sequence alignment is presented in Fig. S7, which also lists the NCBI Refseq accession numbers for each protein. EndoU domains from N. meningitidis MafB1 and B. cereus BC0920 are included for comparison. B. In vitro activities of EndoU toxins. The indicated toxins were purified and incubated with E. coli total RNA in the absence or presence of cognate immunity protein. Reactions were analyzed by Northern blot hybridization with probes to the indicated RNAs. [Colour figure can be viewed at http://wileyonlinelibrary.com]