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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Mol Microbiol. 2018 Aug;109(4):445–457. doi: 10.1111/mmi.13983

Figure 5.

Figure 5

Constraining NT DNA inhibits Exo III cleavage and promotes elongation.

A. U11 ECs were assembled on scaffolds with the full-length (FL) NT DNA or NT DNAs containing deletions of four or five nucleotides. Exo III probing was carried out in the presence and in the absence of RfaH. Numbers indicate the distance from the bubble, to facilitate comparison with Fig. 4.

B. ECs were assembled on scaffolds with the FL or Δ5 NT DNA and [γ32P]-ATP labeled G14 RNA, and incubated with 10 μM GTP, 150 μM ATP, CTP, UTP for 5–240 sec before quenching. RNAs were analyzed on 12% gel. RNA fractions were determined from 3 replicates (±SD).