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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Diagn Microbiol Infect Dis. 2018 Jun 18;92(3):253–258. doi: 10.1016/j.diagmicrobio.2018.06.005

Fig. 5.

Fig. 5

Assessing the cross-reactivity of anti–PenA-peptide antibody against Burkholderia spp. A, Immunoblot on B. multivorans ATTC 17616 blaPenA, B. cenocepacia AU0583 blaPenB, B. pyrrocinia AU1114 blaPenD, B. vietnamiensis AU3578 blaPenE, B. ambifaria AU5203 blaPenF, B. stabilis AU9035 blaPenC, B. dolosa AU9336 blaPenG, B. gladioli AU1009 blaPenN and B. ubonensis AU7314 blaPenH grown to log phase, induced with 1 μg/mL of imipenem, and prepared as crude extracts. B, Clustal Ω sequence alignment of the peptides from the different Pen-like β-lactamases compared with the PenA peptide used for antibody production.