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. Author manuscript; available in PMC: 2019 Mar 24.
Published in final edited form as: Nat Struct Mol Biol. 2018 Sep 24;25(10):928–939. doi: 10.1038/s41594-018-0129-2

Figure 2. Regenerating hepatocytes exhibit pervasive shifts in ribosome occupancies at transcripts encoding RNA processing and metabolic factors.

Figure 2.

Distribution of calculated Fractional Shift Ratios between naïve- and DDC-injured hepatocytes (n = 2 animals/condition) for translating transcripts in a, Monosome, b, Light Polysome (LP), and c, Heavy Polysome (HP) fractions. The null distribution was empirically derived by random separation of the CHOW sample reads into two sets for each fraction. A transcript was called significant (t-test, two sided) when changing in, at least, two fractions with p<0.01, and FDR<0.05 (Benjamini-Hochberg, adjusting for multiple comparisons) in each fraction. SR: Shift ratio. d, Heatmap showing the row normalized fractional shift ratios for the 1486 transcripts changing significantly in their distribution between the three fractions and their corresponding enriched Gene Ontologies (p<0.05) against an expressed gene background set. e, Overlap of mRNA abundance and ribosomal occupancy changes in hepatocytes after DDC-injury.