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. Author manuscript; available in PMC: 2018 Oct 7.
Published in final edited form as: J Comp Neurol. 2017 Jul 24;525(16):3414–3428. doi: 10.1002/cne.24269

FIGURE 7.

FIGURE 7

Algogens and a pruritogen induce Fos in a small number of aromatase-expressing neurons. (a, b, c) Cheek injections of a) capsaicin (5.0 μg/30 μl; 3 males, 3 females), (b) formalin (2% v/v in saline, 50 μl; n = 2 males, 2 females), and (c) chloroquine (200 μg in 50 μl; n = 2 males, 2 females) induced Fos expression in a subset of β-gal+ neurons in the cSTN (arrows). Scale bar (applies to a, b, and c): 100 μm; inset: 20 μm. (d) We did not observe Fos and β-gal overlap in lumbar spinal cord following hindpaw injection of capsaicin (3.0 μg/10 μl). Comparable results were obtained after hindpaw injection of formalin or chloroquine (data not shown). Dashed line outlines border of spinal gray matter. n = 2 males, 2 females. Scale bar: 100 μm. [Color figure can be viewed at wileyonlinelibrary.com]