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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Gastroenterology. 2018 Jun 30;155(4):1218–1232.e24. doi: 10.1053/j.gastro.2018.06.048

Figure 4. Misexpression of TJ proteins is associated with DDC-diet induced chronic liver injury.

Figure 4

(A) RT-PCR analysis shows significant decrease in mRNA expression of claudins-3, 5, 7 and increase in mRNA expression of occludin in DDC-diet for 6das compared to WT mice on normal-diet. Further decrease was seen in claudins-3, 5, 7 level whereas occludin were increased at 14d on DDC-diet. (B) Gel showing PCR product for claudins-3, 5 and GAPDH. (C) In WT liver, claudin-3 and occludin localizes to the membrane in a punctate manner. Localization of claudin-3 to the membrane is reduced in liver of mice fed on DDC-diet for 6d. Occludin levels are more at this stage. At 14d on DDC-diet, claudin-3 continues to decrease whereas occludin levels are still upregulated. (D) Membrane localization of claudin-5 in WT-liver. Membrane localization of claudin-5 is lost in at 6d and 14d on DDC-diet fed mice. * denotes p=0.05

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