Skip to main content
. Author manuscript; available in PMC: 2019 Oct 15.
Published in final edited form as: Bioorg Med Chem. 2018 Apr 7;26(19):5299–5306. doi: 10.1016/j.bmc.2018.04.018

Figure 5.

Figure 5

Peptide 14 does not disrupt pre-formed HSF1 trimers. (A) The SEC profile of wild-type, purified human HSF1 (black line) shows trimer (~160 mL) and monomer (~210 mL) peaks, as previously reported. The isolated trimer was then re-subjected to SEC, confirming it remained a stable sample (blue line). (B) Neither vehicle (DMSO) nor peptide 14 disrupted the HSF1 trimer. (C) SDS-PAGE and Western blots for HSF1 confirm that the protein stays in the trimer fraction after treatment with vehicle (DMSO) or peptide 14.