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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Gastroenterology. 2018 Jun 15;155(4):1177–1191.e16. doi: 10.1053/j.gastro.2018.06.034

Figure 5.

Figure 5

T Cells Are Required for Intestinal Inflammation in R23FR Mice. (A) Representative H&E stained sections from R23FR, R23FR/Rag−/−, R23FR/Mu−/− and R23FR/Tcrd−/− mice at d56. Scale bars, 100 μm. (B) Histological scores of the colon and cecum of R23FR, R23FR/Rag−/−, R23FR/Mu−/− and R23FR/Tcrd−/− mice at d56. Notice that disease was completely absent in R23FR/Rag−/− mice but not in R23FR/Mu−/− and R23FR/Tcrd−/− mice. (C) Schematic representation depicts experimental design for cell depletion experiments. (D) Representative H&E-stained colon and cecum sections (left) and histological scores (right) of R23FR mice after treatment with anti-CD4 and anti-CD8 antibodies. Depletion of CD4+, but not CD8+ T cells significantly reduced disease severity. Scale bars, 100 μm. **p<0.01, *** p<0.001, ns, not significant; by nonparametric Mann-Whitney test.