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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Gastroenterology. 2018 Jun 15;155(4):1177–1191.e16. doi: 10.1053/j.gastro.2018.06.034

Figure 6.

Figure 6

IL23-induced CD4+ T Cells Drive Inflammation in Adoptively Transferred Mice. (A) Experimental setup for adoptive transfer of pooled CD4+ T cells from cecum and mLN of R23FR or FR mice into Rag−/− mice. (B) Representative H&E staining (left) and histological scores (right) of the colon and cecum of Rag−/− mice that received R23FR and FR mice CD4+ T cells fed with different diets. Rag−/− mice that received R23FR CD4+ T cells, but not FR CD4+ T cells, and were fed with 2 cycles of 2019 diet developed severe colitis. Scale bars, 100 μm. *** p<0.001; by nonparametric Mann-Whitney test. (C) Experimental setup for adoptive transfer of CD4+ T cells from large intestine (LI) or colonic mLN (cMLN) of R23FR mice into Rag−/− mice fed with different diets. (D) Representative H&E stained sections (left) and histological scores (right) of the colon and cecum of Rag−/− mice that received mLN or intestinal CD4+ T cells fed with different diets. Scale bars, 100 μm. Both mLN and colonic CD4+ T cells from R23FR mice caused severe colitis in reconstituted Rag−/− mice after a diet switch. *p<0.05, *** p<0.001; by nonparametric Mann-Whitney test.