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. 2018 Oct 7;6(19):e13870. doi: 10.14814/phy2.13870

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© 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Inhibition of CSE stimulates ghrelin secretion. Primary stomach culture and stomach funds tissue were examined by fluorescent immunocytochemistry/immunohistochemistry for ghrelin (green), CSE (red), and nuclei (blue, in merge) (A and B). CSE MW was confirmed by western blot from primary culture cell lysate n = 6 (C). Cells expressing ghrelin, CSE or both were counted in a 100 cell preparation to determine abundance and location within the fundus (D). Acylated ghrelin secretion from primary stomach culture was examined after 4‐h treatments with the CSE inhibitor PPG (E). Cell viability was examined under similar conditions (F). n = 6–9; **P < 0.001 versus control cells.